Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jul 6;8:692540. doi: 10.3389/fcvm.2021.692540

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Liu, Huang, Zhang, Shui, Liu, Wu and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

CXCR7 knockdown reversed the effects of Lacidipine on the in vitro function of ECs under senescence. ECs from the bleomycin + Lacidipine group were transfected with si-CXCR7 or overexpressed P38, respectively. Cell viability was determined using CCK-8 assay (A). Cell senescence was determined through β-galactosidase staining (B). Transwell assay was applied to analyze cell migration ability (C). qPCR was performed to analyze the mRNA levels of VCAM1, Endoglin, MCP-1, and IL-6 (D). Western blot was performed to analyze the protein levels of CXCR7, P38, p-P38, VCAM1, Endoglin, MCP-1, IL-6, P16, and P21 (E,F). (Data are mean ± SD of three independent experiments. *p < 0.05, ***p < 0.001, compared with the bleomycin + Lacidipine group; #p < 0.05, ###p < 0.001, compared with the bleomycin + Lacidipine + Vector group).