Table 3.
Identification of IgE-Bound Gliadin and Glutenin
| Immunoblot | Molecular Mass (kDa) |
Protein |
|---|---|---|
| Gliadin fraction | ||
| L | 30–40 | α/β-gliadin |
| M | 40–59 | γ-gliadin, peroxidase |
| M, H | 45–70 | ω-gliadin |
| Glutenin fraction | ||
| L, M | 40–59 | LMW glutenin, alpha-amylase/subtilisin inhibitor |
| H | 70–100 | HMW glutenin |
Notes: L = proteins in MW range of <40 kDa; MW = Molecular weight; M = proteins in MW range of 40–59 kDa; H = proteins in MW range of >60 kDa.
Abbreviations; kDA, kilodaltons; LMW, low molecular weight; HMW, high molecular weight.