FIGURE 2.

GZD824 suppresses FGFR1‐WT and V561F/M mutant signal pathway in cells (4 h). (A) Effects of GZD824 on FGFR1 signal pathway in H1581, A204, and A549 cancer cell lines. (B) Effects of GZD824 on FGFR1 signal pathway in Ba/F3 model cells. Cells were treated with or without compound GZD824 for 4 h at the indicated concentration, then harvested and lysed. Cell lysates were separated by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and analyzed by western blot for phosphorylated FGFR1, FGFR1, phosphorylated FRS2, FRS2, phosphorylated AKT (Ser473), AKT, phosphorylated Erk, and Erk. GAPDH was used as a control