Figure 8.

Mediator complex-interacting proteins identified in IP-MS. A, Venn diagram showing the overlap between Mediator complex interactors identified by IP-MS, in MED8 (solid line) and MED8ΔQ (dashed line), respectively (see Supplemental Data Set 6 for full lists of interactors). Schematic protein models for MED8 and MED8ΔQ expressed in Arabidopsis cells are presented. The MED8 protein contains a glutamine (Q)-rich domain at the C-terminus with features of IDRs. The Q-rich domain truncation in MED8ΔQ mimics the ceal5 mutation. Numbers indicate the amino acid (aa) position. B, Interactome of the Mediator complex in Arabidopsis. The Mediator complex network was constructed with the Cytoscape software. The gray edges represent interactions detected by our IP-MS experiments, the blue dashed lines indicate interactions reported in plants (String database) and the red edges indicate the one-to-one interaction detected by Maji et al. (2019) within the mediator complex.