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. 2021 Mar 17;33(6):1927–1944. doi: 10.1093/plcell/koab080

Figure 4.

Figure 4

WDL5 driven by the WDL4 promoter does not rescue the delayed hook opening phenotype of wdl4-1 mutant. A, Representative images of microtubules incubated with WDL4 or WDL5 using in vitro assays under low temperature and dilution treatments. Scale bar = 10 μm. B and C, Representative images and quantification of hook curvature in wdl4-1 WDL4pro:GFP-WDL5 complementation lines. Three independent lines were used for analysis. Values in (C) represent mean ± sd (n > 46 independent seedlings; **P < 0.01). Scale bar = 0.5 mm. D and E, Light-triggered rapid hook opening process in WT, wdl4-1, wdl4-1 WDL4pro:WDL4-GFP complementation line (COM #1) and two independent wdl4-1 WDL4pro:GFP-WDL5 lines. Seedlings were germinated and grown vertically in the dark for 42 h, followed by short-term white light treatment as indicated. Representative photographs were taken, and hook curvature was quantified at different time points as labeled. Values in (E) represent mean ± sd (n > 5 independent experiments each containing >10 seedlings; **P < 0.01). Scale bar = 0.5 mm.