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. 2021 Mar 17;33(6):1927–1944. doi: 10.1093/plcell/koab080

Figure 7.

Figure 7

Inactivation of WDL4 impairs trafficking of PIN1–GFP during light-induced hook opening. A and B, Representative images and quantified analysis of BFA effects on light-induced hook opening. WT, wdl4-1, and wdl4-1 WDL4pro:WDL4-GFP (COM #1) seedlings were germinated and grown vertically in the dark for 42 h, and transferred to white light for another 6 h with or without BFA treatment. Values in B represent mean ± sd (n > 36 independent seedlings; **P < 0.01). Scale bar = 0.5 mm. C, WT and wdl4-1 seedlings expressing PIN1pro:PIN1–GFP were germinated and grown vertically in the dark for 42 h, and transferred to white light with CHX pre-treatment (50 μM) for 30 min, followed by CHX + BFA (100 μM) for 1.5 h to induce the formation of BFA bodies. BFA was removed and seedlings were incubated in the presence of CHX and white light for another 30 or 60 min. The images of PIN1–GFP were taken at different time points. The red channel shows auto-fluorescence signals. Scale bar = 20 μm. D, Number of PIN1–GFP-labeled BFA bodies per cell and their relative size. Values represent mean ± sd (n > 14 independent seedlings; *P < 0.05, **P < 0.01).