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. 2021 Mar 25;33(6):1980–1996. doi: 10.1093/plcell/koab090

Figure 1.

Figure 1

Subcellular localization of HYL1 and its various chimeric proteins. A, Subcellular localization of GFP-HYL1 and HYL1-GFP in N. benthamiana leaves. B, Subcellular localization of HYL1-YFP in Arabidopsis protoplasts. C, Subcellular localization of HYL1-GFP in the root of two independent Pro35S:GFP-HYL1/hyl1-2 transgenic lines. D, Immunoblot analysis to detect HYL1 protein in the nuclear and cytoplasmic fractions of WT plant. T, N, and C represent total, nuclear, and cytoplasmic aliquots, respectively. E, Schematic diagrams of various HYL1 chimeric proteins. Grey block, HYL1 double-stranded RNA-binding domain; Yellow oval, NLS of HYL1 (NLS-H); Purple block, PPI domain of HYL1; Red oval, NLS of SV40 (NLS40); Black oval, NES; Green block, GFP protein. F, Localization of various HYL1 fusion constructs from (E) in N. benthamiana leaves. Scale bar, 30 μm in (A) and (F); 5 μm in (B); and 100 μm in (C).