Figure 7.

Effect of cytoplasmic HYL1 on the enrichment of AGO1 in polysomes. A, A diagram showing the sucrose gradients used for the isolation of polysomes from wild-type and ^NLS40HYL1 plants. Sucrose gradients from 50% to 20% were injected into the tubes, and 13 fractions were collected for further analysis. B, The A₂₅₄ absorption profiles of the 13 sucrose gradient fractions. C, Immunoblotting showing the accumulation of HYL1 and AGO1 proteins in the 13 sucrose gradient fractions. * indicates the intensity of the band is ˂0.01 compared to total abundance. D, Immunoblotting showing the accumulation of AGO1 proteins in fractions 8, 9, and 10 of sucrose gradients.