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. 2021 Jul 6;12:633540. doi: 10.3389/fimmu.2021.633540

Figure 1.

Figure 1

GcgR antagonist restores neutrophil migration and inhibits CFU formation but does not alter chemokines and IL-6 production in the peritoneal cavity of diabetic mice after sepsis induction. CLP was performed 21 days after diabetes induction, and the treatment with GcgR antagonist was carried out 24h and 1h before CLP. (A) Analysis of total and differential leukocytes. (B) Bacterial load evaluation. (C–E) Peritoneal levels of CXCL1/KC, CXCL2/MIP-2, and IL-6, respectively. All the analysis were realized 3h after CLP. Each value represents the mean ± S.E.M. For (A, B), each sample value was obtained in blinded and randomized counting. The statistical analysis was performed by one-way ANOVA, followed by Newman–Keuls–Student’s t-test. Results are representative of two independent assays. + P < 0.05 compared to sham non-diabetic mice. & P < 0.05 compared to CLP non-diabetic mice. # P < 0.05 compared to CLP diabetic mice. CFU, Colony-forming unit; CLP, Cecum ligation and puncture; GcgR Ant, GcgR Antagonist.