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. 2021 Jul 6;9:682469. doi: 10.3389/fcell.2021.682469

FIGURE 3.

FIGURE 3

Baicalin promotes the colony formation ability and regeneration capacity of basal cells. (A) Illustration of the use of FACS to obtain basal and luminal cells, 3D culturing of basal and luminal cells under baicalin treatment, and basal cell transplantation assays. (B) The colony formation efficiency of basal cells after treatment with baicalin in Matrigel culture. Scale bar, 50 μm. The red box indicates the focal structure. The data represent three independent experiments. (C) Colony size of basal cells in Matrigel culture. Student’s t-test: **P < 0.01. (D) Number of basal cell colonies after three passages. Student’s t-test: *P < 0.05; ns, not significant. (E) The colony formation efficiency of luminal cells after treatment with baicalin in Matrigel culture. Scale bar, 50 μm. The red box indicates the focal structure. The data represent three independent experiments. (F) Colony size of luminal cells in Matrigel culture. Student’s t-test: ***P < 0.001. (G) Colony number of luminal cells after three passages. Student’s t-test: ***P < 0.001; *P < 0.05. (H) Representative images of fat pads with different percentages of filling. (I) The numbers and sizes (shown as the percentage of fat pad filled) of mammary outgrowths are combined from three independent experiments.