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. 2021 Jul 6;9:643444. doi: 10.3389/fcell.2021.643444

FIGURE 3.

FIGURE 3

Drp1 regulates blood cell homeostasis in Drosophila lymph gland. (A) Whole mount lymph gland showing expression of crystal cell marker ProPO in primary, secondary, and tertiary lobes of control (domeGal4 UAS 2xEGFP) and Drp1 KD (domeGal4 UAS 2xEGFP > UAS Drp1 RNAi) larvae. GFP marks the expression of prohemocyte marker Domeless. Bar diagram shows quantification of mean crystal cell fraction in primary, secondary, and tertiary lobes of indicated genotypes. (B) NRE-GFP (Notch responsive element-GFP) reports activation of Notch signaling in control (domeGal4/ + ; NRE-GFP/ + ; UAS mCD8 RFP/ +) and Drp1 KD (domeGal4/ + ; NRE-GFP/ + ; UAS mCD8 RFP/UAS Drp1 RNAi) lymph gland primary, secondary, and tertiary lobes. RFP marks the expression of prohemocyte marker Domeless. Bar diagram shows quantification of mean NRE-GFP positive (high) cell fraction in primary, secondary, and tertiary lobes of indicated genotypes. (C–E) Bar diagrams show quantification of mean dome > 2xEGFP positive prohemocyte fraction (C), P1 positive plasmatocyte fraction (D) and percentage of lymph glands with lamellocyte differentiation (E) in control and upon Drp1 KD. (F) Schematic summarizes effect of Drp1 on various hemocyte lineages and Notch signaling. n represents number of individual lymph gland lobes analyzed, and N represents number of larvae for each genotype. Scale bar: 100 μm. Error bars represent SEM. Multiple t-test was performed to determine statistical significance. ***P < 0.001, ns: statistically non-significant difference.