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. 2021 Jul 1;22(3):947. doi: 10.3892/etm.2021.10379

Figure 2.

Figure 2

Knockdown of PCAT6 suppresses bladder cancer cell proliferation, migration and invasion. (A) The expression levels of PCAT6 following transfection of siPCAT6 or siNC were determined by reverse transcription-quantitative PCR. (B) MTT and (C) EdU assays were performed to detect cell proliferation after the knockdown of PCAT6. Cell migration ability was examined by (D) wound healing and (E) Transwell assays. Scale bar, 100 µm. (F) Cell invasive ability was analyzed by Transwell assay with Matrigel. Scale bar, 100 µm. Data are presented as the mean ± standard deviation from three independent experiments. *P<0.05 vs. siNC. si, small interfering RNA; NC, negative control; PCAT6, prostate cancer-associated transcript 6.