Figure 4.

Curcumin inhibits the malignant phenotypes of PCa cells via miR-30a-5p. (A) After transfection of miR-30a-5p inhibitors and curcumin treatment, PCLAF expression in PC-3 cells and DU145 cells was detected via western blotting. (B) BrdU assay was used to detect the proliferation of PCa cells. (C) Wound healing and (D) Transwell assays were used to detect the migration and invasion of PCa cells. (E) Effects of curcumin and miR-30a-5p inhibitors on the expression of apoptosis-related proteins were detected via western blotting. ^*P<0.05, ^**P<0.01, ^***P<0.001. PCa, prostate cancer; miR, microRNA; PCLAF, PCNA clamp associated factor.