TABLE 2.
Analysis of DNA methylation patterns in regenerated plants of five generations compared with donor plants.
| Description of patten | Class |
Banding patten |
G1 | G2 | G3 | G4 | G5 | |||
|
G0 |
G1-G5 |
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| HpaII | MspI | HpaII | MspI | |||||||
| No Change | A | 1 | 0 | 1 | 0 | 122 ± 12 | 58 ± 1 | 56 ± 1 | 47 ± 1 | 57 ± 8 |
| B | 0 | 1 | 0 | 1 | 178 ± 2 | 129 ± 3 | 143 ± 4 | 129 ± 2 | 130 ± 5 | |
| C | 1 | 1 | 1 | 1 | 400 ± 12 | 419 ± 20 | 412 ± 14 | 425 ± 18 | 406 ± 19 | |
| D | 0 | 0 | 0 | 0 | 58 ± 5 | 48 ± 4 | 44 ± 3 | 44 ± 3 | 37 ± 3 | |
| Total percentage (%) | 758 ± 5 80.69 ± 0.57 |
655 ± 18 69.76 ± 1.87 |
656 ± 15 69.83 ± 1.61 |
645 ± 16 68.69 ± 1.67 |
630 ± 15 67.09 ± 1.57 |
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| Demethylation | E | 1 | 0 | 1 | 1 | 59 ± 2 | 126 ± 13 | 127 ± 15 | 133 ± 18 | 132 ± 14 |
| F | 0 | 1 | 1 | 1 | 24 ± 5 | 64 ± 9 | 70 ± 1 | 81 ± 6 | 84 ± 5 | |
| G | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | |
| H | 0 | 1 | 1 | 0 | 1 ± 1 | 15 ± 9 | 4 ± 2 | 3 ± 1 | 6 ± 2 | |
| I | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | |
| J | 0 | 0 | 0 | 1 | 18 ± 2 | 25 ± 2 | 24 ± 4 | 25 ± 3 | 30 ± 2 | |
| Total percentage (%) | 102 ± 5 10.86 ± 0.56 |
230 ± 15 24.49 ± 1.60 |
224 ± 17 23.89 ± 1.82 |
242 ± 15 25.81 ± 1.58 |
252 ± 19 26.80 ± 2.00 |
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| Methylation | K | 1 | 1 | 1 | 0 | 25 ± 1 | 15 ± 2 | 19 ± 5 | 9 ± 1 | 17 ± 3 |
| L | 1 | 1 | 0 | 1 | 15 ± 3 | 7 ± 3 | 9 ± 2 | 8 ± 2 | 16 ± 9 | |
| M | 1 | 1 | 0 | 0 | 3 ± 2 | 2 | 3 ± 2 | 1 ± 1 | 4 ± 2 | |
| N | 1 | 0 | 0 | 1 | 2 ± 1 | 4 ± 1 | 5 ± 2 | 7 ± 3 | 6 ± 1 | |
| O | 1 | 0 | 0 | 0 | 16 ± 5 | 9 ± 3 | 13 ± 3 | 13 ± 2 | 8 ± 3 | |
| P | 0 | 1 | 0 | 0 | 18 ± 4 | 17 ± 3 | 10 ± 3 | 14 ± 1 | 7 ± 1 | |
| Total percentage (%) | 79 ± 4 8.45 ± 0.40 |
54 ± 6 5.75 ± 0.59 |
59 ± 4 6.28 ± 0.44 |
52 ± 5 5.50 ± 0.58 |
57 ± 13 6.11 ± 1.38 |
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