Figure 2: Site density affects early activation and memory phenotype of CD22 CART cells.

(A) Site density cell lines were co-incubated with CD22 CART for 24 hours, and PD1 expression was stained flow cytometry and analyzed on Fortessa flow machine. (B) CD22 CAR was co-cultured in vitro with CD22^lo or Nalm6 leukemia for 8 days and cells were evaluated using flow cytometry and analyzed on Fortessa flow machine. (C) Cells were extracted from CD22 CAR treated CD22^lo or Nalm6 NSG mice 16 or 30 days after tumor injection. Cells were stained for flow cytometry and analyzed on Fortessa flow machine. (D) Cells were extracted from CD22 CAR treated CD22^lo or Nalm6 NSG mice 16 days following tumor injection. Cells were stained for flow cytometry and analyzed on Fortessa flow machine. This data was reproducible across two separate experiments. Statistics were calculated using Mann-Whitney test (**** p < 0.0001, * p < 0.0332).