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. 2021 Jul 6;12:680328. doi: 10.3389/fendo.2021.680328

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Copyright © 2021 Li, Wang, Li and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of M2 macrophages and exosomes derived from M2 macrophages. (A) Expression of M2 marker CD206 in polarized RAW 264.7 cells analyzed by flow cytometry. (B) Morphology identified by TEM, scale bar:100 nm. (C) The surface biomarkers CD63, CD81 were analyzed by Western blotting assay. (D) Size distribution profiles of M2D-Exos was detected by nanoparticle tracking analysis. (E) The exosomes derived from M2 macrophages was marked with red fluorescence dye PKH26 and co-cultured with BMSCs, scar bar = 50 µm.