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. 2021 Jul 6;12:680328. doi: 10.3389/fendo.2021.680328

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Copyright © 2021 Li, Wang, Li and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Exosomes derived from M2 macrophages inhibited adipogenic differentiation of BMSCs. (A, B) Adipogenic proteins, C/EBPβ and PPARγ, were measured by western blotting analysis and quantified. Control group was BMSCs treated in adipogenic inducer. (C) Osteogenic genes, C/EBPβ and PPARγ, were measured by RT-PCR analysis. Control group was BMSCs treated in adipogenic inducer. (D) Oil red O staining was used to measure the formation of lipid droplets in BMSCs after treated by PBS (control group) or M2D-Exos for 14 days. Scale bar = 50 µm. (E) The statistical data of Oil red O staining. *p < 0.05 compared to the control group. Control group was BMSCs treated in adipogenic inducer.