Effects of miR-133b on growth of TNBC cells. RT-PCR was used to test the expression of miR-133b in TNBC cells of each group (A); CCK-8 method was used to examine its influence on the proliferation level of TNBC cells (B, C); Colony formation test and flow cytometry were used to examine its influence on colony formation and apoptosis of TNBC cells (D, E); Western blot analysis was used to examine its influence on the Wnt-β-catenin pathway of TNBC cells (F, G). Note: *P<0.05, **P<0.01 vs. miR-NC or between groups; #P<0.05 vs. miR-133b. Abbreviation: miR, microRNA; NC, negative control; TNBC, triple negative breast cancer; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; PI, propidium iodide; Wnt1, wingless-type MMTV integration site family, member1; FOXQ1, Forkhead box Q1.