Table 2.
Gene primer sequences and conditions used for qRT-PCR
| Gene | NCBI RefSeq | Primer sequence (5’-3’) (Forward/Reverse) | Melting temperature (°C) | Optimal concentration |
|---|---|---|---|---|
| ACT-β | KR710455 | CCCTCCATCGTCCACCGCAAATGCT | 79.1 | 50 nM |
| CTGCTGTCACCTTCACCGTTCCAGT | 73.2 | 50 nM | ||
| IL-6 | HUMIFNB2A | ATAACCACCCCTGACCCAA | 55.9 | 50 nM |
| CCATGCTACATTTGCCGAA | 53.4 | 50 nM | ||
| TGF-β | NM_000660 | CTCCCGCAAAGACTTTTCCCCAGACCT | 64.0 | 300 nM |
| CCACGGAAATAACCTAGATGGGCGCGAT | 63.7 | 300 nM | ||
| IDO | M58159 | CATCCTGATTCCTGCAAGCC | 56.3 | 50 nM |
| TCTGCTATGATAAAATGTGCTCT | 52.0 | 50 nM | ||
| TNF | AB202113 | CCTGAAAACAACCCTCAGACGCCACA | 63.0 | 300 nM |
| TCCTCGGCCAGCTCCACGTCCC | 67.8 | 300 nM | ||
| MMP13 | NM_002427 | CCAGAACTTCCCAACCGTATTGATGC | 72.3 | 50 nM |
| TGCCTGTATCCTCAAAGTGAACAGC | 69.1 | 50 nM | ||
| COL2a1 | X16711 | CCCATCTGCCCAACTGACC | 58.5 | 50 nM |
| CACCTTTGTCACCACGATCCC | 58.2 | 50 nM | ||
| iNOS | AF045478 | AACGTTGCTCCCCATCAAGCCCTT | 63.3 | 50 nM |
| AGCAGCAAGTTCCATCTTTCACCCACT | 62.3 | 50 nM |