Skip to main content
. 2021 Jun 15;13(6):6076–6086.

Figure 4.

Figure 4

miR-889-3p targets the LDHA mRNA 3’-UTR. A. Bioinformatics tools (miRanda, miRBase, TargetScan) indicated that LDHA may function as the target protein of miR-889-3p. B. LDHA mRNA 3’-UTR containing miR-889-3p complementary sites was constructed, which were named as LDHA-WT or LDHA-Mut. C. Luciferase reporter assay showed the closely connection within miR-889-3p and LDHA mRNA. D. RT-PCR showed the LDHA mRNA expression in HCC cells (HepG) transfected with miR-889-3p mimics and circUBE2D2 overexpression. C, D. Experiments were performed in triplicate and were repeated three times. E. Pearson correlation analysis of LDHA and circUBE2D2 was detected in HCC tissues cohort (30 samples). **P<0.01 vs. control.