Knockdown of Trem1 ameliorated spinal cord neuronal apoptosis after SCIRI. A. BBB score evaluated mice motion after 30, 60 and 120 min of reperfusion in sham mice and SCIRI mice with or without Trem1 shRNA adenovirus. ***P<0.001. B. TUNEL staining was performed on slices from spinal cord specimens. Scale bar: 100 μm. C. Immunoblotting analysis of Trem1, Caspase-3, Bax and Bcl2 in sham mice and SCIRI mice with or without Trem1 shRNA adenovirus. D. Immunoblotting analysis of pAKT, PI3K, p-p65, TNF-α, p-SYK and SYK in sham mice and SCIRI mice with or without Trem1 shRNA adenovirus.