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. 2021 Jun 15;13(6):5911–5927.

Figure 6.

Figure 6

miR-1278 directly targets LATS1. A. Schematic representation of the predicted binding site of miR-1278 in the 3’UTR of LATS1 mRNA (LATS1-3’UTR-WT), and the mutant LATS1 3’UTR designed by us (LATS1-3’UTR-MUT). B. The luciferase activity was determined using the Dual-Luciferase Reporter System in BCPAP. C. The luciferase activity was determined using the Dual-Luciferase Reporter System in TPC-1. D. Western Blot and statistical results showing the expression of LATS1 in BCPAP and TPC-1 transfected with miR-1278 mimics or miR-NC. GAPDH was used as an internal reference. E. Western Blot and statistical results showing the expression of LATS1 in BCPAP and TPC-1 transfected with miR-1278 inhibitor or NC inhibitor. GAPDH was used as an internal reference. Statistical results were presented as the mean ± SME. Two-tailed paired t-test, *P<0.05, **P<0.01, ***P<0.001.