Table 2.

Primers used for each gene expression analysis by real-time RT.

Genes	Primer sequence (5’-3’)	Accession number	Amplicon size	Efficiency
					ef-1α	Fw: 5’-ATGCCCCCAAGTTCCTGAAG-3’	NM_001124339.1	140	1.95
Rv: 5’-AACAGCAACAGTCTGCCTCA-3’
il-1	Fw: 5’-TGAGAACAAGTGCTGGGTCC-3’	NM_001124347.2	148	2.02
	Rv: 5’-GGCTACAGGTCTGGCTTCAG-3’
lysozyme	Fw: 5’-TGCCTGTCAAAATGGGAGTC-3’	NM_001124716.1	152	1.89
	Rv: 5’-CAGCGGATACCACAGACGTT-3’
mIgM	Fw: 5’-AAAGCCTACAAGAGGGAGACCGAT-3’	X65263.1	128	2
	Rv: 5’-AGAGTTATGAGGAAGAGTATGATGAAGGTG-3’
mcsf-ra	Fw: 5’-ATCTCCACTCATGGCGACACA-3’	AB091826	177	2
	Rv: 5’-CATCGCACTGGGTTTCTGGTA-3’
mpo	Fw: 5’-GCAGAGTCACCAATGACACCA-3’	GBTD01119227	68	2
	Rv: 5’-ATCCACACGGGCATCACCTG-3’
il-10	Fw: 5’-CCGCCATGAACAACAGAACA-3’	NM_001245099.1	105	1.98
	Rv: 5’-TCCTGCATTGGACGATCTCT-3’
tgf-β1	Fw: 5’-GCCAAGGAGGTCCACAAGTT-3’	NM_001281366.1	146	2.06
	Rv: 5’-GTGGTTTTGATGAGCAGGCG-3’
cd4-2β	Fw: 5’-AAGCCCCTCTTGCCGAGGAA-3’	AY899932	108	2
	Rv: 5’-CTCAACGCCTTTGGTACAGTGA-3’
vapA	Fw: 5’-ATTAGCCCGAACGACAACAC-3’	KP184543.1	148	2.02
	Rv: 5’-CCAACACAATGAAACCGTTG-3’

Primers were designed according to Cornet et al. (41) and Khuyen et al. (42).