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. 2021 Jul 20;16(1):190–199. doi: 10.1038/s41396-021-01059-w

Table 2.

Enzymes used to alter Symbiodiniaceae cell-surface molecules and carbohydrates used to mask E. diaphana cell-surface lectins, prior to inoculation experiments.

Organism Treatment Product # Concentration Enzyme specificity
Symbiodiniaceae α-amylase A6255 5 mg/ml α-(1,4) glycan linkages
Symbiodiniaceae Trypsin T6567 6 μg/ml Peptides on the C-terminal side of lysine and arginine residues
E. diaphana D-galactose G0750 10 mM /
E. diaphana Methyl-α-D-galactose G1000 10 mM /
E. diaphana Methyl-β-D-galactose G1125 10 mM /
E. diaphana D-glucose 47829 10 mM /
E. diaphana D-mannose M6020 10 mM /
E. diaphana D-galacturonic acid 48280 10 mM /
E. diaphana D-glucuronic acid G5269 10 mM /
E. diaphana L-fucose F2252 10 mM /
E. diaphana L-rhamnose W373011 10 mM /
E. diaphana D-xylose PHR2102 10 mM /

All products were purchased from Sigma-Aldrich.