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. 2021 Jul 6;8:607585. doi: 10.3389/fmed.2021.607585

Figure 3.

Figure 3

Methyltransferase-like 3 (METTL3) knockdown (using METTL3 siRNA) inhibits inflammatory cytokines in human and rat fibroblast-like synoviocytes (FLSs). (A) METTL3 protein levels in TNF-α-treated rheumatoid arthritis (RA)-FLSs after METTL3 knockdown, as detected by Western blotting. (B) METTL3 mRNA levels in TNF-α-incubated RA-FLSs after METTL3 silencing, as detected by real-time quantitative PCR (qPCR). (C) IL-6 mRNA levels in TNF-α-incubated RA-FLSs with METTL3 knockdown, as detected by qPCR. (D) The number of IL-6 in TNF-α-incubated RA-FLS supernatants were analyzed by ELISA. (E) Western blotting was used to measure METTL3 protein levels in TNF-α-incubated AIA-FLSs with METTL3 siRNA. (F) METTL3 mRNA levels in TNF-α-incubated AIA-FLSs after using METTL3 siRNA, as detected by qPCR. (G) IL-6 mRNA levels in TNF-α-incubated AIA-FLSs with METTL3 knockdown, as detected by qPCR. (H) ELISA was used to detect the production of IL-6 in TNF-α-incubated AIA-FLS supernatants. All of these values are presented as the mean ± SD. NC, negative control. **P < 0.01 vs. the control group. #P < 0.05, ##P < 0.01 vs. the NC group.