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. 2021 Jul 6;8:607585. doi: 10.3389/fmed.2021.607585

Figure 4.

Figure 4

Methyltransferase-like 3 (METTL3) overexpression along with high expression of inflammatory cytokines. (A) METTL3 protein levels in TNF-α-incubated rheumatoid arthritis fibroblast-like synoviocytes (RA-FLSs) with METTL3 overexpression, as detected by Western blotting. (B) METTL3 mRNA levels in TNF-α-incubated RA-FLSs after overexpressing METTL3, as detected by real-time quantitative PCR (qPCR). (C) IL-6 mRNA levels in TNF-α-incubated RA-FLSs after overexpressing METTL3, as detected by qPCR. (D) The number of IL-6 in TNF-α-incubated RA-FLS supernatants were analyzed by ELISA. (E) METTL3 protein level was measured by Western blotting in TNF-α-incubated AIA-FLSs after METTL3 overexpression. (F) METTL3 mRNA level was detected by qPCR in TNF-α-incubated AIA-FLSs after using METTL3-PEX. (G) IL-6 mRNA levels were detected by qPCR in TNF-α-incubated AIA-FLSs with overexpressed METTL3. (H) The number of IL-6 in TNF-α-incubated AIA-FLS supernatants were measured by ELISA after transfection. All of these values are presented as the mean ± SD. NC, negative control. *P < 0.05, **P < 0.01 vs. the control group. ##P < 0.01 vs. the NC group.