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. 2021 Jul 6;8:607585. doi: 10.3389/fmed.2021.607585

Figure 7.

Figure 7

Methyltransferase-like 3 (METTL3)-RNAi inhibits migration and invasion in fibroblast-like synoviocytes (FLSs). (A) Western blotting was used to measure MMP3 and MMP9 protein levels after METTL3 silencing in rheumatoid arthritis (RA)-FLSs. (B) MMP3 and MMP9 mRNA levels were detected by real-time quantitative PCR (qPCR) in TNF-α-incubated RA-FLSs after transfection with METTL3-RNAi. (C) Representative photograph of TNF-α-treated, METTL3-knockdown RA-FLSs 24 h after wound formation (original magnification, ×10) and quantitative analysis of the wound healing extent. (D) After METTL3 silencing for 24 h in TNF-α-incubated RA-FLSs, Transwell invasion assay was photographed (original magnification, ×10), and quantitative analysis of the cell number. (E) Western blotting was used to measure MMP3 and MMP9 protein levels after METTL3 silencing in TNF-α-incubated adjuvant-induced arthritis (AIA)-FLSs. (F) qPCR was used to analyze MMP3 and MMP9 mRNA levels in TNF-α-incubated AIA-FLSs after transfection with METTL3-RNAi. (G) After METTL3 silencing in TNF-α-incubated AIA-FLSs, photographed after wound formation at 24 h (original magnification, ×10) and quantitative analysis of the wound healing extent. (H) After METTL3 silencing in TNF-α-incubated RA-FLSs, Transwell invasion assay was photographed (original magnification, ×10), and quantitative analysis of the cell number. All of these values are presented as the mean ± SD. NC, negative control. **P < 0.01 vs. the control group. #P < 0.05, ##P < 0.01 vs. the NC group.