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. 2020 May 27;7(11):1776–1786. doi: 10.1093/nsr/nwaa110

Figure 3.

Figure 3.

Effect of GmSWEET10a on seed size, fatty-acid content and protein content. (a) Genotype of the sw10a mutant edited by the CRISPR/Cas9 system. The arrow indicates the target site of the CRISPR/Cas9 editing in the region of exon 3 of GmSWEET10a. Changes in the DNA sequence in the targeted region and the amino-acid sequence of the sw10a mutant are highlighted in red. Numbers inside the brackets indicate the number of amino acids coded by the sequence. (b) Increased expression of GmSWEET10a was achieved in transgenic soybean lines OE-10a-1 and OE-10a-2 by introducing additional copies of the GmSWEET10a genomic sequence into the Williams 82 cultivar. (c) Seed appearance of the sw10a mutant, OE-10a-1 and OE-10a-2. Scale bars, 1 cm. (d)–(f) 100-seed weight (d), fatty-acid content (e) and protein content (f) of mature seeds from wild-type (WT), sw10a mutant, OE-10a-1 and OE-10a-2. DW, dry weight. Data are means ± s.d. ((d) n = 10; (e) and (f) n = 5). *P < 0.05; **P < 0.01 (Student's t-test).