Skip to main content
. Author manuscript; available in PMC: 2021 Sep 16.
Published in final edited form as: Bioconjug Chem. 2020 Sep 2;31(9):2179–2190. doi: 10.1021/acs.bioconjchem.0c00365

Figure 3.

Figure 3.

Mass spectral analysis of B2036-Alkyne. (A) Deconvoluted intact high-resolution mass spectrum of B2036-Alkyne. (B) Sequence coverage map of B2036-Alkyne was determined following digestion of the purified protein with trypsin and identification of the resulting peptides via LC/ESI/MS/MS. Sequence coverage was determined to be 88.5% (black bolded text) with 100% sequence match and includes coverage of the incorporated noncanonical amino acid propargyl tyrosine (green). (C) Sample tandem mass spectrum of the peptide ADRLNQLAFDTYQEFEEA(pglY)IPK with m/z = 2699.30 in LC/ESI/MS analysis following trypsin digestion of B2036-Alkyne with assigned y- and b- series ions (blue and red, respectively).