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Site-specific PEGylation of B2036-Alkyne. (A) Scheme of conjugation of PEG-azide to B2036-Alkyne via CuAAC. SDS-PAGE of purified site-specific B2036-PEG conjugates stained with (B) Coomassie to visualize protein and (C) 0.1 M iodine to visualize PEG. Lane 1: protein standards; lane 2: B2036-Alkyne; lane 3: B2036–5k mPEG; lane 4: B2036–10k mPEG; lane 5: B2036–20k mPEG.
