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. 2020 Dec 16;53(3):819–828. doi: 10.4143/crt.2020.1013

Fig. 2.

Fig. 2

Combination treatment promotes suppression of cell viability and induction of apoptotic cell death in ovarian cancer cells. SKOV-3 and OVCAR-3 cells were treated with either prexasertib (0–100 μM) or rucaparib (0, 10, and 50 μM) for 72 hours after cells were seeded. (A) Cell viability was by PrestoBlue in SKOV-3 and OVCAR-3 cells. (B) Apoptotic cell analysis was measured using an Annexin V assay by fluorescence activatdetermineded cell sorting. (C) Caspase-3 activity was measured by luciferase assay using Caspase-Glo 3/7 reagent. (D) Representative images of immunoblotting data for levels of checkpoint kinase 1 (Chk1), poly(ADP-ribose) polymerase (PARP), cleaved caspase-3, and cleaved PARP proteins in combination treatment conditions. Alpha-tubulin was used as a loading control. *p < 0.05 compared to 0 μM, **p < 0.01 compared to 0 μM, ***p < 0.001 compared to 0 μM, #Control group. Values are expressed as the mean±standard deviation.