Fig. 3.

Combination treatment increases mitotic entry of sub G1 phase cells and activates the DNA damage signaling pathway. (A) Cell-cycle analysis was determine using FxCycle propidium iodide (PI)/RNase staining solution by fluorescence activated cell sorting in both SKOV-3 and OVCAR-3 cell lines. (B) Representative images of immunoblotting data for levels of phospho-Ser345 checkpoint kinase 1 (Chk1), phospho-Ser296 Chk1, Rad51, γH2AX, and phosphor-Ser10 Histone H3 proteins in combination treatment conditions. Alpha-tubulin was used as a loading control. Values are expressed as the mean±standard deviation. **p < 0.01 compared to 0 μM, ***p < 0.001 compared to 0 μM, ^#Control group.