FIGURE 5:

Representative images of axonal/dendritic morphology (SMI31 and SMI32) in thalamic perivascular and subependymal areas of demyelination and normal appearing grey matter (NAGM). Above panels represent 3,3 ′-diaminobenzidine (DAB) immunohistochemistry of perivascular (top left) and subependymal (bottom left) areas of demyelination and adjacent NAGM. A blood vessel (V) and the third ventricle (asterisk “*”) are labeled. Demyelinated areas have reduced axonal/dendritic density, particularly in subependymal areas compared to perivascular. Areas of axonal swelling/blebs are apparent. Below panel are confocal micrographs of demyelinated (left) and normal appearing (right) thalamus. Some axons appear swollen and irregular (white arrow) in demyelinated regions.