Table 2.
Gradient elution program of RP-HPLC.
| Time (min) | Solution A (%) | Solution B (%) | Solution C (%) | Flow rate (mL/min) |
|---|---|---|---|---|
| 0 | 85 | 6 | 9 | 1 |
| 10 | 80 | 8 | 12 | 1 |
| 25 | 70 | 15 | 15 | 1 |
| 40 | 45 | 25 | 30 | 1 |
| 50 | 45 | 25 | 30 | 1 |
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