Skip to main content

View full-text article in PMC

. 2021 Jul 16;13:447–457. doi: 10.2147/BCTT.S317682

Table 1.

Temperature Optimization for Each Primer

Primer		Sequences	T* (^oC)
Caspase-9	Forward	5ʹ-AAGTGACCCTCCCAAGTAGC-3’	60.5
Caspase-9	Reverse	5ʹ-GTTCTGGCCAGGTCTCTTCT-3’	60.5
Caspase-3	Forward	5ʹ-AAAATACCAGTGGAGGCCGA-3’	58.4
Caspase-3	Reverse	5ʹ-GCACAAAGCGACTGGATGAA-3’	58.4
PARP-1	Forward	5ʹ-TGGAACATCAAGGACGAGCT-3’	60.5
PARP-1	Reverse	5ʹ-CATCGCTCTTGAAGACCAGC-3’	60.5
Bcl-2	Forward	5ʹ-TCCTCTTTACACTGGCCAGG-3’	60.5
Bcl-2	Reverse	5ʹ-GAGTATTTGTGCAGCGAGGG-3’	60.5
GAPDH	Forward	5ʹ-AAGGTGAAGGTCGGAGTCAAC-3’	57.3
GAPDH	Reverse	5ʹ-CTTGATTTTGGAGGGATCTCG-3’	57.3

Note: *T, annealing temperature.