Table 1.
Summary of CRISPR-based plant precise gene editing systems.
| Species | Transformation | Donor | Promoter | Nuclease | Target | Selection | GT efficiency | Reference |
|---|---|---|---|---|---|---|---|---|
| Arabidopsis | Agrobaterium | BeYDV replicons | PcUBI | Cas9/Cas9n | GL1 | None | 0.0% | [105] |
| T-DNA | Cas9 | 0.1% | ||||||
| Arabidopsis | Biolistic | CaLCUV replicons | 35S | ZFN | ADH1 | None | 4.3% | [79] |
| Arabidopsis | Agrobaterium | T-DNA | PcUBI | Cas9/Cas9 | ADH1 | Allyl alcohol | 0.1% | [75] |
| Arabidopsis | Agrobaterium | T-DNA | PcUBI | Cas9/Cas9n | ALS | Imazapyr | 0.14–0.3% | [82] |
| AtYao | 0.1% | |||||||
| AtEC1.1/1.2 | 0.05–0.97% | |||||||
| Arabidopsis | Agrobaterium | T-DNA | AtDD45 | Cas9 | ROS1 | None | 6.3–8.3% | [81] |
| DME | 5.3–9.1% | |||||||
| Tobacco | Agrobaterium | BeYDV replicons | 35S | TALEN/Cas9 | ALS | Kanamycin | [79] | |
| ZFN | GUS | NA | ||||||
| Tomato | Agrobaterium | BeYDV replicons | 35S | Cas9 | ANT1 | Kanamycin | 3.65–11.66% | [106] |
| TALEN | 4.67–9.65% | |||||||
| T-DNA | TALEN | 1.3% | ||||||
| Tomato | Agrobaterium | BeYDV replicons | SlUBI10 | Cas9 | ctisto | None | 25.0% | [107] |
| Soybean | Biolistic | DNA vector | EFLA2 | Cas9 | DD20 | Hygromycin | 4.6% | [108] |
| DD45 | Hygromycin | 3.8% | ||||||
| Potato | Agrobaterium | BeYDV replicons | 35S | Cas9 | ALS | Kanamycin | 32.2% | [109] |
| TALEN | 34.5% | |||||||
| Maize | Biolistic | DNA vector | ZmUBI | Cas9 | ALS2 | Chlorsulfuron | 0.2–0.4% | [76] |
| ssDNA | ||||||||
| Agrobaterium | T-DNA | LIG1 | Bialaphos | 2.5–4% | ||||
| ALS | Chlorsulfuron | 4.2% | ||||||
| Rice | Biolistic | DNA vector | ZmUBI | Cas9 | ALS | Bispyribac | 90.6% | [77] |
| Agrobaterium | 75.0% | |||||||
| Rice | Agrobaterium | T-DNA | 35s | Cas9 | ALS | Bispyribac | 0.147–1% | [110] |
| Rice | Agrobaterium | WDV replicons | ZmUBI | Cas9 | GST | 19.4% | [42] | |
| ACT1 | 7.7% | |||||||
| T-DNA | GST | 6.8% | ||||||
| ACT1 | 0.0% | |||||||
| Rice | Agrobaterium | Chimeric sgRNA | OsUBI | Cas9 | ALS | Bispyribac | 2.1% | [111] |
| Rice | Biolistic | DNA vector | 35S | FnCas12a | CAO | Hygromycin | 3–8% | [112] |
| LbCas12a | 0–3% | |||||||
| Rice | Biolistic | DNA vector | ZmUBI | Cas9 | NRT1.1B | None | 6.7% | [78] |
| Rice | Biolistic | DNA vector | ZmUBI | LbCas12a | ALS | Bispyribac | 11.1% | [113] |
| Maize | Biolistic | DNA vector | ZmUBI | Cas9 | ARGOS8 | None | 0.9% | [114] |
| Wheat | Biolistic | WDV replicons | ZmUBI | Cas9 | MLO | GFP | 3.2–6.4% | [115] |
| EPSPS | 4.7% |
BeYDV: bean yellow dwarf virus; CaLCUV: cabbage leaf curl virus; WDV: wheat dwarf virus; ssDNA: single-stranded DNA; PcUBI: parsley ubiquitin promoter; SlUBI10: tomato ubiquitin10 promoter; EFLA2: soybean elongation factor gene promoter; ZmUBI: maize ubiquitin promoter.