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. 2019 Jan 24;6(1):87–100. doi: 10.1093/nsr/nwz002

Figure 1.

Figure 1.

Generation of BMAL1 knockout cynomolgus monkeys. (A) Designated PAS.3 domain and the respective sequences in BMAL1 exon 13 that were targeted in cynomolgus monkeys. Red, sgRNA1 and 3 sequences. (B) Summary of all BMAL1-edited monkeys in this study. (C) Sequence alterations in the five CRISPR/Cas9-edited mutant monkeys, examined via PCR amplification of BMAL1 exon 13, followed by sequence analysis. (D) Images of male A5 (BMAL1-WT), male A6 (BMAL-KO), female A7 (BMAL1-WT), and female A3 (BMAL1-KO) cynomolgus monkeys at 4 months of age. (E) Expression of WT BMAL1 transcript over zeitgeber time (ZT) 2, 8, 14 and 20 for BMAL1-WT versus BMAL1-KO monkeys, shown by average blood mRNA levels. (F) Immunoblots of BMAL1 and PER2 levels in major tissues collected from stillbirths A1 and A9. GAPDH was used as the reference. *P < 0.05, **P < 0.01 and ***P < 0.001; Student's t-test.