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. 2021 Jul 16;16:2105–2118. doi: 10.2147/COPD.S311222

Figure 1.

Figure 1

Circ-RBMS1 is highly expressed in COPD patients and CSE-induced 16HBE cells. (A) Detection of circ-RBMS1 expression level in blood samples of non-smokers, smokers and smokers with COPD using qRT-PCR. (B) qRT-PCR analysis of circ-RBMS1 expression in 16HBE cells exposed to 1.5%, 3%, and 4.5% CSE for 24 h. (C) qRT-PCR analysis of circ-RBMS1 expression in 16HBE cells treated with RNase R or Mock. (D) The expression of circ-RBMS1 and linear RBMS1 mRNA by qRT-PCR in reverse transcription using Random and Oligo(dT)18 primers. (E) qRT-PCR indicating the distribution of circ-RBMS1 in the cytoplasmic and nuclear fractions of 16HBE cells. **P<0.01, ***P<0.001, ****P<0.0001.