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. 2021 Jul 16;16:2105–2118. doi: 10.2147/COPD.S311222

Figure 2.

Figure 2

Circ-RBMS1 knockdown reversed CSE-induced apoptosis, inflammation and oxidative stress in 16HBE cells. (A) CCK-8 assay for the viability of 16HBE cells treated with 1.5%, 3%, and 4.5% CSE for 24 h. (BI) 16HBE cells were pretreated with 3% CSE for 24 h, followed by transfection with si-circ-RBMS1 or si-NC. (B) qRT-PCR analysis of circ-RBMS1 expression in 16HBE cells. (C) CCK-8 assay for 16HBE cell viability. (D) EDU assay for the DNA replication of 16HBE cells. (E) Flow cytometry for 16HBE cell apoptosis. (F) ELISA analysis for the levels of IL-1β and TNF-α in 16HBE cells. (G and H) Detection of MDA and SOD levels in 16HBE cells using commercial kits. (I) Western blot analysis of Bax and Bcl-2 protein levels in 16HBE cells. **P<0.01, ***P<0.001, ****P<0.0001.