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. 2021 Jul 16;16:2105–2118. doi: 10.2147/COPD.S311222

Figure 7.

Figure 7

MiR-197-3p attenuates CSE-induced apoptosis, inflammation and oxidative stress in 16HBE cells by targeting FBXO11. (A) Western blot analysis of FBXO11 protein level in 16HBE cells transfected with FBXO11 or pcDNA. (B–I) 16HBE cells were pretreated with 3% CSE for 24 h, followed by transfection with miR-NC, miR-197-3p, miR-197-3p + pcDNA, miR-197-3p + FBXO11. (B) Western blot analysis of FBXO11 protein level in 16HBE cells. (C) CCK-8 assay for 16HBE cell viability. (D) EDU assay for the DNA replication of 16HBE cells. (E) Flow cytometry for 16HBE cell apoptosis. (F) ELISA analysis for the levels of IL-1β and TNF-α in 16HBE cells. (G and H) Detection of MDA and SOD levels in 16HBE cells using commercial kits. (I) Western blot analysis of Bax and Bcl-2 protein levels in 16HBE cells. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.