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. 2018 Nov 29;6(3):438–451. doi: 10.1093/nsr/nwy150

Figure 3.

Figure 3.

Domain organization and structures of Cas9 and its complexes. (a) Domain organization of Cas9. (b) Crystal structure of Apo-SpCas9 (PDB: 4CMP). (c) Crystal structure of SpCas9 in complex with sgRNA (PDB: 4ZT0). (d) Cartoon showing the sgRNA observed in the structure of the SpCas9-sgRNA binary complex. (e) Crystal structure of SpCas9 in complex with sgRNA and target DNA (5′-NGG-3′ PAM) (PDB: 4UN3). (f) Cartoon showing the working model of type II-A SpCas9 (PDB: 4UN3). (g) The complex structure of SpCas9-sgRNA-AcrIIA4 (PDB: 5XBL). sgRNA and AcrIIA4 are colored orange and cyan, respectively. In panels (b, c, e and f), SpCas9 domains are colored the same as panel (a).