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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Mol Cell. 2020 Sep 14;80(1):87–101.e5. doi: 10.1016/j.molcel.2020.08.017

Figure 6. Consequences of Akt isoform deletion in the neutrophils of tumor-bearing mice.

Figure 6.

A. The effect of Akt1 deletion on metastasis in MRP8-Cre mice after orthotopic transplantation of E0771 cells. Upper panel: Schematic of experimental design. Bottom panel: Quantification of metastasis. B. The effect of Akt1 deletion on neutrophils in the lungs of MRP8-Cre tumor-bearing mice (n=8). Upper panels show representative lung section images stained with anti-Ly6G. Data are presented as the means ±SEM. P<0.0001, for metastatic nodules and neutrophils using an unpaired t test. C. The effect of G-CSF on the survival of neutrophils isolated from control, and systemically deleted Akt1 and Akt2 tumor-bearing mice. Data are presented as the mean ± SEM. p=0.0055 for Akt1f/f;R26CreERT2 versus R26CreERT2 and p=0.099 for Akt2f/f;R26CreERT2 versus R26CreERT2. D. The effect of G-CSF on the level of MCL1 in neutrophils isolated from control and systemically deleted Akt1 and Akt2 tumor-bearing mice.