KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Monoclonal rabbit anti-pan Akt (clone C67E7) | Cell Signaling Technology | Cat. # 4691 |
| Monoclonal rabbit anti-Akt1 (clone C73H10) | Cell Signaling Technology | Cat. # 2938 |
| Monoclonal rabbit anti-Akt2 (clone D6G47) | Cell Signaling Technology | Cat. # 3063 |
| Polyclonal rabbit anti-pSer473 pan Akt | Cell Signaling Technology | Cat. # 9271 |
| Polyclonal rabbit anti-pSer308 pan Akt | Cell Signaling Technology | Cat. # 9275 |
| Monoclonal rabbit anti-pSer473 Akt1 (clone D7F10) | Cell Signaling Technology | Cat. # 9018 |
| Monoclonal rabbit anti-pSer474 Akt2 (clone D3H2) | Cell Signaling Technology | Cat. # 8599 |
| Monoclonal rabbit anti-PRAS40 (clone D23C7) | Cell Signaling Technology | Cat. # 2691 |
| Monoclonal rabbit anti-phospho PRAS40 (clone C77D7) | Cell Signaling Technology | Cat. # 2997 |
| Monoclonal rabbit anti-GSK3-beta (clone D5C5Z) | Cell Signaling Technology | Cat. # 12456 |
| Monoclonal rabbit anti-p-GSK3-beta (clone D85E12) | Cell Signaling Technology | Cat. # 5558 |
| Monoclonal rabbit anti-GAPDH (clone 14C10) | Cell Signaling Technology | Cat. # 2118 |
| Monoclonal rabbit anti-b-Actin (clone 13E5) | Cell Signaling Technology | Cat. # 4970 |
| Monoclonal rat anti-Ly6G (clone 1A8) | Biolegend | Cat. # 127601 |
| Monoclonal rabbit anti-Ki67 (clone SP6) | Abcam | Cat. # ab16667 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Xylenes (Histological), Fisher Chemical | Fisher Scientific | Cat. # X3S-4 |
| Hematoxylin Solution, Harris Modified | Sigma-Aldrich | Cat. # HHS32–1L |
| Eosin Y solution, alcoholic | Sigma-Aldrich | Cat. # HT110116 |
| Recombinant Mouse G-CSF Protein | R&D Systems | Cat. # 414-CS-025 |
| Pierce™ ECL Western Blotting Substrate | Thermo Fisher Scientific | Cat. # 32106 |
| Pierce™ Protease Inhibitor Mini Tablets | Thermo Fisher Scientific | Cat. # A32953 |
| Pierce™ Phosphtase Inhibitor Mini Tablets | Thermo Fisher Scientific | Cat. # A32957 |
| HyClone™ Dulbecco’s High Glucose Modified Eagles Medium | Fisher Scientific | Cat. #SH30022.01 |
| HyClone™ RPMI 1640 Media | Fisher Scientific | Cat. #SH30027.01 |
| HyClone™ Phosphate Buffered Saline (PBS) | Fisher Scientific | Cat. #SH30256.01 |
| Propidium iodide | Sigma-Aldrich | Cat. # P4170–25MG |
| Hoechst 33342, trihydrochloride, trihydrate | Thermo Fisher Scientific | Cat. # H3569 |
| Histopaque®−1077 | Sigma-Aldrich | Cat. # 10771–100ML |
| Histopaque®−1119 | Sigma-Aldrich | Cat. # 11191–100ML |
| Gibco™ ACK Lysing Buffer | Fisher Scientific | Cat. # A1049201 |
| Corning Matrigel Matrix | Corning Life Sciences | Cat. # 354230 |
| Collagenase, Type 4 | Worthington | Cat. #LS004188 |
| Tamoxifen | Sigma-Aldrich | Cat. #T5648–5G |
| Corn Oil | Sigma-Aldrich | Cat. #C8267–500ML |
| MK-2206 | Shelleck Chem | Cat. #S1078 |
| Canagliflozin (CANA) | MedChem Express, Monmouth Junction, NJ, USA | |
| MILLIPLEX MAP Mouse Metabolic Hormone Magnetic Bead Panel | Millipore Sigma | Cat. # MMHMAG44K |
| Deposited Data | ||
| Raw and analyzed data | This paper | GEO: GSE135096 |
| Original data for figures in this study | This paper | http://dx.doi.org/10.17632/77t55d5zjf.1 |
| Experimental Models: Cell Lines | ||
| E0771 mouse breast cancer cells | ATCC | ATCC® CRL-3461 |
| Experimental Models: Organisms/Strains | ||
| Mouse FVB/N: MMTV-NIC | W.J. Muller (McGill University) | N/A |
| Mouse FVB/N: MMTV-Erbb2 | W.J. Muller (McGill University) | N/A |
| Mouse FVB/N: Tg(MMTV-PyVT)634Mul/J (MMTV-PyMT) | The Jackson laboratory | JAX: 002374 |
| Mouse: FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J (LSL-Luc) | The Jackson laboratory | JAX:005125 |
| Mouse FVB/N: Rosa26(R26)CreERT2 knock-in mice (strain 01XAB) | Wang et al., 2016 | N/A |
| Mouse FVB/N: Akt1f/f | Wang et al., 2016 | N/A |
| Mouse FVB/N: Akt2f/f | Wang et al., 2016 | N/A |
| Mouse FVB/N:Akt1f/f;R26RCreERT2 | Wang et al., 2016 | N/A |
| Mouse FVB/N:Akt2f/f;R26RCreERT2 | Wang et al., 2016 | N/A |
| Mouse FVB/N: MMTV-NIC;Akt1f/f | This paper | N/A |
| Mouse FVB/N: MMTV-NIC;Akt2f/f | This paper | N/A |
| Mouse FVB/N: MMTV-Erbb2;Akt1f/f;R26RCreERT2 | This paper | N/A |
| Mouse FVB/N: MMTV-Erbb2;Akt2f/f;R26RCreERT2 | This paper | N/A |
| Mouse FVB/N: MMTV-NIC;Akt1f/f;LSL-Luc | This paper | N/A |
| Mouse FVB/N: MMTV-NIC;Akt2f/f;LSL-Luc | This paper | N/A |
| Mouse FVB/N: MMTV-PyMT;Akt1f/f;R26RCreERT2 | This paper | N/A |
| Mouse FVB/N: MMTV-PyMT;Akt2f/f;R26RCreERT2 | This paper | N/A |
| Mouse: MRP8-Cre-ires/GFP, Mrp8creTg | The Jackson laboratory | JAX: 021614 |
| Mouse: MRP8-Cre;Akt1f/f | This paper | N/A |
| Mouse: MRP8-Cre;Akt2f/f | This paper | N/A |
| Mouse: (NOD)/Shi-scidIL-2Rγnull (NOG) | The Jackson laboratory | JAX:016117 |
| Software and Algorithms | ||
| GraphPad Prism 6.0 | GraphPad Inc. | https://www.graphpad.com/scientificsoftware/prism/ |
| Image J | Schneider et al., 2012 | https://imagej.nih.gov/ij/ |
| Seurat | https://satijalab.org/seurat/ | |
| R-3.3.2 | R Core Team (2013). | https://cran.rproject.org/ |