Figure 3.

Protein binding to a receptor within a solid-state nanopore. (A) Finite-element model of the nanopore and its material composition. (B) Histogram of simulated and experimental binding event durations. The rate constants k_a and k_d used for simulating binding were taken from ref (59) (light blue bars) or inferred from measurements of ref (35) (dark blue bars). The experimental τ_off data (red line) are from ref (35). The transmembrane voltage is 200 mV. (C) Calculated and experimental values for rate constant k_off at different voltages. The color code is as in panel B. Straight lines are least-squares fits to symbols of the same color.