Table 2. Corrected number of cells at stage 8 of the seminiferous epithelium cycle (SEC), spermatogenic indexes, Sertoli cells number and spermatic reserve of the testis of Wistar rats with Walker 256 tumor and supplemented with L-glutamine.
| Parameters | Experimental groups | |||
|---|---|---|---|---|
| Sertoli cell | 16.08±1.48Aa | 17.32±0.90Ca | 13.02±0.91Bc | 17.36±1.45Cd |
| Type A spermatogonia | 4.60±0.30 | 3.88±0.39 | 4.46±0.34 | 5.58±1.43 |
| Primary spermatocyte at preleptotene-leptotene | 26.72±3.82Aa | 35.46±2.49Cb | 35.06±1.59Bc | 37.50±2.32Cc |
| Primary spermatocyte at pachytene | 50.50±1.35 | 51.14±2.01 | 49.00±6.61 | 44.20±5.63 |
| Round spermatid | 189.38±7.07 | 191.20±12.84 | 179.74±15.74 | 173.14±18.57 |
| Mitotic index | 5.85±1.04 | 9.16±0.70 | 7.88±0.46 | 7.04±1.62 |
| Meiotic index | 3.75±0.12 | 3.74±0.27 | 3.69±0.25 | 3.96±0.55 |
| Spermatogenic yield | 41.37±3.84 | 49.68±6.67 | 40.67±6.47 | 32.74±8.93 |
| Sertoli cell support capacity | 16.95±1.67Aa | 16.30±2.20Ca | 20.67±2.03Bc | 15.06±1.44Cd |
| Sertoli cell number per testis (1011) | 8.64±0.10 | 7.53±0.85 | 5.67±0.79 | 10.10±1.65 |
| Sertoli cell number per gram of testis (1011) |
3.04±0.55Aa | 2.80±0.16Ca | 2.10±0.33Bc | 3.67±0.72Dd |
| Spermatic reserve of the testis (107) | 1.01±0.03 | 1.10±0.09 | 0.78±0.09 | 1.00±0.14 |
| Spermatic reserve per gram of testis (106) |
3.56±0.44 | 4.11±0.43 | 2.88±0.37 | 3.64±0.58 |
C: Control; CG: Control supplemented with L-glutamine, WT: Walker 256 tumor; WTG: Walker 256 tumor supplemented with L-glutamine. Averages with different pairs of capital letters on the same line (A-B; C-D) show comparisons between control (C x WT) and supplemented groups (CG x WTG), respectively. Averages with different pairs of lower case letters on the same line (a-b; c-d) show comparisons between control (C x CG) and Walker Tumor groups (WT x WTG), respectively. Data are expressed as mean ± standard deviation of the mean (p≤0.05).