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. 2020 Sep 24;11(1):972–983. doi: 10.1080/21655979.2020.1814658

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© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — miR-301a-3p inhibits Eca-109 cell proliferation.

GFP expression in Eca-109 cells transfected with adenovirus vectors expressing miR-301a-3p inhibitor (301ai group) or negative control inhibitor (NCi group). (A) The relative level of miR-301a-3p/U6 in NCi group and 301ai group (B). MTT assay (C) and colony formation assay (D) in NCi group and 301ai group. The relative protein levels of p-AKT/AKT, BCL-2, and BAX in NCi group and 301ai group (E).*p < 0.05; **p < 0.01 vs NCi group; n = 3.