Figure 3.

LINC00265 functions as a ceRNA for miR-144 in GC cells. (a) The distribution of LINC00265 within GC cells was determined by the nuclear/cytoplasmic fractionation assay. (b) The wild-type miR-144-binding sequences in LINC00265, as predicted by starBase 3.0. The mutations in the LINC00265 sequence that disrupt the interaction between LINC00265 and miR-144 are shown too. (c) NCI-N87 and KATO III cells that were transfected with either agomiR-144 or agomir-NC were harvested and analyzed for miR-144 expression by RT-qPCR. *P < 0.05 vs. the agomir-NC group. (d) Luciferase reporter assays were performed on NCI-N87 and KATO III cells that were transfected with either agomiR-144 or agomir-NC and either LINC00265-wt or LINC00265-mut. *P < 0.05 vs. group agomir-NC. (e) The expression profile of miR-144 in the 40 pairs of GC tissues and adjacent-normal-gastric tissue samples was analyzed by RT-qPCR. *P < 0.05 vs. the normal tissues. (f) Expression of miR-144 in NCI-N87 and KATO III cells transfected with either si-LINC00265 or si-NC was determined by RT-qPCR. *P < 0.05 vs. the si-NC group