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. 2020 Jul 14;11(1):769–778. doi: 10.1080/21655979.2020.1791590

Figure 1.

Figure 1.

Effect of shRNA or plasmid transfection on MUC1 expression in LSCC cells. (a) MUC1 protein expression was detected in Hep2, TU-212, TU686, TU177 and AMC-HN-8 cells by Western blot assay; (b) MUC1 mRNA was detected in Hep2, TU-212, TU686, TU177 and AMC-HN-8 cells by RT-PCR assay; (c) TU686 cells were transfected into MUC1 shRNA 1, 2, and 3 for 72 h, MUC1 protein expression was detected by Western blot assay; (d) MUC1 mRNA expression was detected by RT-PCR assay; E, Hep2 cells were transfected into pcDNA3.1 or pcDNA3.1 MUC1 for 72 h, MUC1 protein expression was detected by Western blot assay. vs untreated.**P < 0.01.