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. 2020 Aug 31;11(1):932–938. doi: 10.1080/21655979.2020.1809922

Figure 3.

Figure 3.

Circ-LRP6 sponges miR-182. (a, b) RNA pull-down assay in two ESCC cells using biotin-labeled circ-LRP6 probe, followed by qRT-PCR analysis. (c) The binding site of miR-182 on circ-LRP6. (d) Luciferase reporter assay in two ESCC cells using wild-type or mutant circ-LRP6 vector. (e) qRT-PCR analysis of miR-182 expression after circ-LRP6 knockdown. (f, g) CCK-8 and Transwell assays testing cell viability and invasion in circ-LRP6-silenced ESCC cells transfected with miR-182 inhibitors. *p < 0.05, **p < 0.01.