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. 2021 Feb 19;12(1):682–696. doi: 10.1080/21655979.2021.1887645

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — BCAR4 modulates miR-139-3p/ELAVL1 pathway in ESCC cells. (a). BCAR4 wide-type (BCAR4-wt) and the mutated-type (BCAR4-mut) sequences in the has-miR-139-3p binding sites were shown according to DIANA-LncBase v2. (b). Relative expression of miR-139-3p was examined using RT-PCR assay after miR-139-3p overexpression. n = 3, **p < 0.01, ***p < 0.001, compared with the miR-NC group. (c). BCAR4 knockdown increased the expression of miR-139-3p in both EC109 and TE-1 cells. n = 3, *p < 0.05, ***p < 0.001, compared with the shCtrl group. (d). The dual luciferase reporter assay showed that overexpression of miR-139-3p repressed relative luciferase activity of EC109 cells transfected with BCAR4-wt or BCAR4-mut. n = 6, ***p < 0.001, compared with the miR-NC group. (e). The viability of EC109 and TE-1 cells in BCAR4 knockdown or BCAR4 plus miR-139-3p knockdown groups was detected by MTT assay. n = 3, ***p < 0.001, compared with the Ctrl group; ^##p < 0.01, compared with the shBCAR4 group. (f). The migration cells of EC109 and TE-1 cells in BCAR4 knockdown or BCAR4 plus miR-139-3p knockdown groups was detected by Transwell assay. n = 3, ***p < 0.001, compared with the Ctrl group; ^##p < 0.01, ^###p < 0.001, compared with the shBCAR4 group. (g). ELAVL1 wide-type (ELAVL1-wt) and the mutated-type (ELAVL1-mut) sequences in the has-miR-139-3p binding sites were shown according to DIANA-microT-CDS. (h). The dual luciferase reporter assay showed that overexpression of miR-139-3p or BCAR4 knockdown repressed relative luciferase activity of EC109 cells transfected with ELAVL1-wt. n = 6, ***p < 0.001, compared with the miR-NC group or shCtrl. (i). miR-139-3p overexpression reduced the expression of ELAVL1 in both EC109 and TE-1 cells. n = 3, **p < 0.01, compared with the miR-NC group. (j). BCAR4 knockdown reduced the expression of ELAVL1 in both EC109 and TE-1 cells. n = 3, **p < 0.01, compared with the shCtrl group. (k). MTT assay was using to detect the cell viability of ESCC cells after transfecting indicated plasmids. n = 3, **p < 0.01, ***p < 0.001